1. If the mutation creates or destroys a restriction site then this can be simply examined in the products of the reaction.

2. Several deletion mutants were prepared by exploiting singular restriction sites in the cDNA to enable progressive sequencing.

3. Only relevant restriction sites are indicated in the expanded regions.

4. Incomplete restriction sites produced on the junctions with linkers and adapters are indicated by enzymes with asterisks.

5. BglII, XhoI and BamHI restriction sites are at equivalent positions in each of the repeats as shown.

6. In order to connect with PUC19 vector, the gene fragments of heat shock protein HSP70 were linked with the double-stranded artificial joints With EcoR I restriction site.