1. In this study, a detailed restriction map was constructed using multiple groups of restriction endonuclease analysis.

2. Restriction map A map of a segment of DNA showing the cleavage sites of restriction endonucleases and their physical distance apart, usually measured in base pairs.

3. The restriction map of R2 gene was construed for further use.

4. The restriction map of the recombinant was analysed and compared with that of human SRY gene.

5. The terminal fragment hybridization method reported here could overcome this difficulty, and the restriction map can be elucidated easily by using labe...