1) Histopathologic tissue diagnosis should always be attempted, followed by total thyroidectomy, radioiodine, or external beam radiation, and chronic thyroid-stimulating hormone suppression.

2) The levels of serum free Triiodothyronine (FT3) and free Thyroxine (FT4) with thyroid-stimulating hormone (TSH) were measured by CLIA and RIA methods respectively.

3) The method of screening for congenital hypothyroidism using radioimmunoassay (RIA) of thyroid-stimulating hormone (TSH) in dried blood spot was reported in this study.

4) Thyroid-stimulating hormone releasing hormone (TRH) stimulates the anterior pituitary to liberate the thyroid-stimulating hormone and prolactin.

5) Methods:The peripheral blood from calcar pedis of the 72-hour 28 088 newborns were tested in quantity of thyroid-stimulating hormone(TSH) by the time-resolve immunoassay.