1 Methods:Amidase activities of PCA from AAV were measured in diverse experimental conditions by respective method of chromogenic substrate and active partial thromboplastin time(APTT).

2 A novel enantioselective amidase screening system was developed and proved to be efficient and accurate.

3 Kinetic parameters of a cyclic amidase with maleimide as the substrate was assayed by HPLC method.

4 Penicillin-G amidase enzyme is one of the enzyme outside the cell, which is secreted by the cell and which is from Bacillus Megaterium through second fermentation.

5 The amidase of Nocardia sp. is one of important industrial enzymes.

6 The carrier of pink diatomite (6201), a kind of inorganic materials, was used to immobilize penicillin amidase.

7 In specific embodiments, the invention provides methods for inducing and stabilizing nitrile hydratase activity, amidase activity, and asparaginase I activity.