1, However, the molecular mechanism of ameloblast differentiation remains unclear.

2, The exudation of enamel matrix almost stopped in ameloblast secretion phase.

3, In the cap stage, odontoblast, ameloblast and the adjacent dental papilla expressed the OSX protein strongly. OSX protein also existed in enamel-dentinal junction, the new enamel and dentinal tubule.

4, BMP was expressed in the ameloblast, the intermedium stratum cell and the stellate reticulum cell but not in dental papillae cells or odontoblast.

5, The exudation of enamel matrix almost stop in ameloblast secretory phase.

6, These results indicate that MAPK signaling functions, at least in part, as the inducer of ameloblast differentiation.

7, RESULTS: In early age of tooth development, MEPE was expressed in ameloblast,(http:///ameloblast.html) odontoblast and pulp tissue.

8, Ameloblastin is a specific enamel matrix protein. It is synthesized and secreted by ameloblast and distributed around the enamel rods.

9, The expression of odontoblast significantly decreased in the late bell stage, while ameloblast showed positive expression.

10, Objective:The main idea of this study was to explore the method of the normal ameloblast culture in vitro.