1. When the induced hemolymph was heated, the supernate had inhibitive effect on bacteria, but had no effect on fungi.

2. The supernate of virus culture was used as templet in overlapping PCR, then the interesting gene was obtained.

3. In addition, a reduced concentration of 5-HT in the supernate of endo-toxin group was also observed.

4. The results showed that both the cell-free extract and culture supernate from mouse SRS-82 cell line were leukemogenic.

5. Though there was no difference in detection rate with the three antigens, the cell culture supernate antigen was superior to other two antigens in titre and line sharpness.

6. There was also secretion of recombinant goat FSH in culture media supernate using radioimmunoassay.