1. Produce hybridoma cell, isolation and purify antibody.

2. Results Ascites preparation is influenced by mice, immunosuppressor, hybridoma, ascites collection and disposal, and so on.

3. The principles of hybridoma technique are involved in some advantages, high specificity, sensitivity, convenience and quickness.

4. Objective To prepare celluloseby hybridoma technique, and to establish I - ELISA method for clinical application.

5. Conclusion The hybridoma cell line may be applicable to the diagnosis of HBsAg.

6. Then the hybridoma cell lines secreting high titer of McAbs were screened.

7. By this procedure, Milstein and Kohler developed a hybridoma that made a specific antibody.

8. The hybridoma cell strain E 3 E 6 secreting monoclonal antibodies was screened and clo ned by indirect ELISA.

9. Conclusion Plasmodial aldolase has been successfully expressed and purified, and the established hybridoma cell lines can secrete McAbs specific to the aldolase of P. falciparum.