1. The sensitivity of soybean cultivars to Agrobacterium tumefaciens is commonly low and there is significant difference among genotypes.

2. A method Agrobacterium tumefaciens transformation for perennial ryegrass was developed using the calli derived from mature embryos, and the transgenic plants were successfully obtained.

3. Biotype 3 Agrobacterium tumefaciens strain MI3-2 from grapevine in nature is responsible for the formation of tumors on a very narrow host range of plants, particularly vitis(grapevine)cultivars.

4. Several wheat cultivars were transformed in planta by Agrobacterium tumefaciens and numbers of transformants were gained.

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5. Crown gall strains caused by Agrobacterium tumefaciens S-1 702, C-58 have been established from sunflower hypocotyls, tobacco stems and stem pithes, carrot roots and potato tubers.

6. The transformation system mediated by Agrobacterium tumefaciens is one of the most widespread transgenic method for plants.

7. These recent advance in gene engineering, with optimization of factors affecting genetic transformation of potato via Agrobacterium tumefaciens, were reviewed and discussed in this paper.

8. Based on this highly efficient shoot induction, the application of filter paper to the genetic transformation of Artemisia annum L. by Agrobacterium tumefaciens was investigated.

9. We genetic transformation of ACO antisense gene into lily's bulb and leafstalk by Agrobacterium tumefaciens - mediated method.

10. The crown gall disease is a bacterial disease induced by Agrobacterium tumefaciens.

11. In this study, the foreign gene was transferred into wheat immature embryos by Agrobacterium tumefaciens mediated, and the factors influencing wheat transformation were analyzed.