(1) Hematoxylin and eosin staining and safranin O staining were used to observe the secretion of cartilage matrix.

(2) Safranin O staining showed strong positive reaction, suggested that there were rich glycosaminoglycan in matrix.

(3) They do not fade or damage specimens. Examples include fast green and the counterstain safranin. See also negative staining.

(4) Light green A stain sued in light microscopy to show up cytoplasm and cellulose. It is commonly used as a counterstain with safranin to highlight cytoplasm. See staining.

(5) Gram negative bacteria (e. g. Cyanobacteria) are decolorized, and may be counterstained with other dyes, such as safranin, carbol fuchsin, neutral red.