1, A method for determination of auramine O in legume -food by high performance . liquid chromatography-diode array detector (DAD) was studied.

2, Objective To investigate the possibility of auramine O fluorescent dyes in specific stain of pathology.

3, Conclusion Auramine O fluorescence method is worth manipulating and popularizing in specific stain practice.

4, Methods Biopsy tissue considered tuberculosis was stained by fluorescent dyes made with auramine O and compared to classic Ziehl - Neelsen acid-fast stain.

5, METHODS: The oocyst of Cryptosporidium parvum in 5421 fresh stool samples from eleven areas of Anhui province was tested by auramine phenol stain and improved anti acid stain respectively.