1, The cortical granules were arranged beneath the oolemma membrane.

2, The sperm has fused to the egg cell membrane (oolemma) prior to becoming incorporated almost completely into the egg. The zona pellucida has been removed in this preparation.

3, Increased motility, zona binding and oolemma fusion capacities are also acquired within the epididymis and are necessary for those cells that finally arrive at the site of fertilization.

4, In maturing oocytes, CGs began to arrange themselves beneath the oolemma, and mitochondria dispersed toward the central region of cytoplasm.

5, In the peripheral ooplasm there are many cortical specializations rods, which are perpendicular to the oolemma and located in the cortical crypt formed by the subsided vitelline envelope.

6, CONCLUSION: HBV DNA sequences were able to pass through the zona and oolemma to enter into oocytes and integrate into their chromosomes.