1. OBJECTIVE To find a new way to synthesize scopoletin.

2. METHODS Using assisted microwave irradiation to synthesize scopoletin .

3. Objective To determine the content of scopoletin in the roots and stalks of herba Argyreia seguinii by HPLC.

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4. The preparation method can effectively produce the Scopoletin industrially on a large scale.

5. Objective:To investigate the interaction of major constituents in Ipomoea Pescaprae(scopoletin and hesperetin).

6. The fluorescence quantum yields of scopoletin and scopolin were measured to be 0.71 and 0.21 respectively, using quinine sulfate as a reference.

7. OBJECTIVE To establish a highperformance liquid chromatography (HPLC) method for the determination of scopoletin in Tengluoning capsules.

8. OBJECTIVE To develop a RP-HPLC method to determine the content of scopoletin in Cortex Lycii from different sources.

9. It was found that solution acidity had great effect on the fluorescence spectra of scopoletin, and had no effect on scopolin.

10. OBJECTIVE: To establish an HPLC method for the determination of Scopoletin in Xuelong capsules.

11. Five parts were included in this thesis:1. Fluorescence spectra of scopoletin and scopolin in CE were studied.